Detection of hepatitis C virus RNA by a reliable, optimized single-step reverse transcription polymerase chain reaction
✍ Scribed by C. Payan; T. Dupré; L. Bélec
- Book ID
- 116158434
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 647 KB
- Volume
- 146
- Category
- Article
- ISSN
- 0923-2516
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Three PCR methods based on the GB virus-C/ hepatitis G virus (GBV-C/HGV) 5ЈUTR and NS3 genomic region were used for the detection of GBV-C/HGV RNA in serum of 62 patients with chronic hepatitis C virus (HCV) infection. Ten of 62 (16%) patients were found to have GBV-C/ HGV RNA, which was confirmed b
Although detection of hepatitis C virus RNA with polymerase chain reaction has become the standard for diagnosis, extensive application has been thwarted by polymerase chain reaction's labor intensiveness, risk of false-positive results through contamination and time required for individual assays.