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Single-step reverse transcription-polymerase chain reaction for hepatitis C virus RNA with DNA enzyme immunoassay hybridization

✍ Scribed by Christopher Payan; Francis Bloch; Isabelle Gaultier; Laurent Bélec

Book ID
113256505
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
570 KB
Volume
53
Category
Article
ISSN
0166-0934

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✍ Hsiang Ju Lin; Naiyi Shi; Masashi Mizokami; F. Blaine Hollinger 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 701 KB

## Abstract A procedure is described for the detection of hepatitis C virus (HCV) RNA in blood by means of the polymerase chain reaction (PCR) in which the reverse transcription step and two rounds of amplification are carried out in a single tube. This results in fewer manipulations, reduced risk