𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of frequent allelic loss of 6q23–q25.2 in microdissected human breast cancer tissues

✍ Scribed by Hiroaki Fujii; Weibo Zhou; Edward Gabrielson

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
469 KB
Volume
16
Category
Article
ISSN
1045-2257

No coin nor oath required. For personal study only.

✦ Synopsis

Detection of alfelic loss in human breast cancer is hindered by the fact that breast cancer tissues are frequently infiltrated by stromal and inflammatory cells. For this study, we carefully microdissected infiltrating breast cancer tumor cells from contaminating normal cells and analyzed the DNA from these samples for allelic loss on the long arm of chromosome 6 by using a panel of 15 dinucleotide repeat markers. We found 53 of the 66 cases rrudied (80%) t o have allelic loss of either the entire chromosomal arm (37 cases) or a portion of the chromosomal arm ( I 6 cases). One common region that was identified for all tumors with deletions of 6q was the area between markers D65310/314 and D65473/255, consistent with a tumor suppressor gene locus at 6q23-6q25.2. The use of tissue microdissection allowed the detection of allelic loss,in this chromosomal region .in human breast cancer at a much higher frequency than was previously recognized.

📜 SIMILAR VOLUMES

Association of allelic losses on human c
Association of allelic losses on human chromosomal arms 11q and 16q in sporadic breast cancer
✍ Rita K. Schmutzler; Rolf Fimmers; Erhard Bierhoff; Barbara Lohmar; Anke Homann; 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 French ⚖ 517 KB

Breast-carcinoma development presumably results from multiple mutational events in tumor-associated genes. Certain results indicate that some tumor-suppressor genes may combine their pathogenetic potential to synergistically promote tumor growth. In an effort to identify such mechanisms in breast tu

Identification of a 100-kb region of com
Identification of a 100-kb region of common allelic loss on chromosome bands 10q25–q26 in human endometrial cancer
✍ Hiromitsu Yamakawa; Satoru Nagase; Michihiro Yuki; Hiromi O. Shiwaku; Toru Furuk 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 193 KB 👁 1 views

In human endometrial cancer, we have previously identified a 790-kb region of common allelic loss in chromosome bands 10q25-q26, flanked by D10S587 and D10S1723. We constructed a contig covering the entire deleted region using YACs, PACs, and BACs. Five overlapping cosmid clones derived from YAC clo

Identification of a 910-Kb region of com
Identification of a 910-Kb region of common allelic loss in chromosome bands 16q24.1–q24.2 in human lung cancer
✍ Masami Sato; Yuriko Mori; Akira Sakurada; Shinichi Fukushige; Yuichi Ishikawa; E 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 395 KB 👁 2 views

To understand the molecular pathogenesis of human lung cancer, we analyzed allelic deletions on the long arm of chromosome 16 by PCR amplification of microsatellite markers. A total of 203 lung cancer specimens (78 squamous cell carcinomas and 125 adenocarcinomas) were analyzed. In both cell types,

Mapping of a new target region of alleli
Mapping of a new target region of allelic loss to a 6-cM interval at 21q21 in primary breast cancers
✍ Kenji Ohgaki; Aritoshi Iida; Fujio Kasumi; Goi Sakamoto; Masao Akimoto; Yusuke N 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 90 KB 👁 1 views

A detailed analysis of loss of heterozygosity (LOH) in breast cancers was performed with 11 microsatellite markers on the long arm of chromosome 21. Among the 142 tumors examined, 44 (31%) showed LOH at one or more loci. Peak LOH frequency was observed on band 21q21. Deletion mapping identified a ne

Mapping of a new target region of alleli
Mapping of a new target region of allelic loss to a 2-cM interval at 22q13.1 in primary breast cancer
✍ Aritoshi Iida; Keisuke Kurose; Rie Isobe; Futoshi Akiyama; Goi Sakamoto; Masatak 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 95 KB 👁 2 views

Allelic losses on chromosome arm 22q are frequently observed in human meningiomas and in carcinomas of the colon, ovary, and breast. Among 140 primary breast cancers we examined for loss of heterozygosity (LOH) at 16 polymorphic loci on the long arm of chromosome 22, 56 (40%) showed LOH for at least