Identification of a 100-kb region of common allelic loss on chromosome bands 10q25–q26 in human endometrial cancer

✍ Scribed by Hiromitsu Yamakawa; Satoru Nagase; Michihiro Yuki; Hiromi O. Shiwaku; Toru Furukawa; Kousuke Yoshinaga; Eiichi Soeda; Masato Hoshi; Yutaka Hayashi; Shinji Sato; Akira Yajima; Akira Horii

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 193 KB
• Volume: 23
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199809)23:1<74::aid-gcc12>3.0.co;2-e

✦ Synopsis

In human endometrial cancer, we have previously identified a 790-kb region of common allelic loss in chromosome bands 10q25-q26, flanked by D10S587 and D10S1723. We constructed a contig covering the entire deleted region using YACs, PACs, and BACs. Five overlapping cosmid clones derived from YAC clones completely covered the entire deleted region: its size was estimated to be no larger than 200 kb. We further performed two-color fluorescence in situ hybridization (FISH) analysis to confirm the deletion and narrowed down the deleted region to 100 kb or less; it was covered by three overlapping cosmid clones that were included in one BAC clone. Restriction endonuclease mapping identified a region in which NotI, SalI, SmaI, and XhoI were clustered, suggesting the possible existence of a CpG island.