𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of flavivirus antibodies in human serum by epitope-blocking immunoassay

✍ Scribed by Donald S. Burke; Ananda Nisalak; Mary Kay Gentry

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
487 KB
Volume
23
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Human flavivirus group-reactive, dengue complex-reactive, and encephalitis virus complex-reactive antibodies were detected using epitope-blocking immunoassays in which the binding of selected mouse monoclonal antibodies to flavivirus antigens was blocked by human serum. When late (> 6 months after illness) convalescent sera were tested, the epitope-blocking immunoassays were superior to the hemagglutination inhibition test and comparable to the plaque reduction neutralization for identifying subjects immune to dengue, to Japanese encephalitis, or both viruses.

πŸ“œ SIMILAR VOLUMES

A highly sensitive enzyme immunoassay of
A highly sensitive enzyme immunoassay of anti-insulin antibodies in human serum
✍ Takeyuki Kohno; Eiji Ishikawa; Satoru Sugiyama; Syuji Nakamura; Yoshimasa Kanema πŸ“‚ Article πŸ“… 1987 πŸ› John Wiley and Sons 🌐 English βš– 431 KB

A highly sensitive enzyme immunoassay who had been treated with insulin for 0.6of anti-insulin antibodies in human serum 24 months. The detection limit of anti-inis described. Serum samples were sub-sulin IgG in human serum was 1,000 to jected to successive processes of the in-3,000-fold less than t

Sensitive detection of tumor marker CA15
Sensitive detection of tumor marker CA15-3 in human serum by capillary electrophoretic immunoassay with chemiluminescence detection
✍ Yan-Ming Liu; Yan-Li Zheng; Jun-Tao Cao; Yong-Hong Chen; Fu-Rong Li πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 448 KB

## Abstract A new and sensitive non‐competitive immunoassay (IA) for tumor marker carbohydrate antigen 15‐3 (CA15‐3) by CE coupling with ECL detection has been developed. This method is based on luminol–H~2~O~2~ reaction catalyzed by horseradish peroxidase (HRP). The optimum CE separation and CL de

An enzyme labelled nuclear antigen immun
An enzyme labelled nuclear antigen immunoassay for detection of cytomegalovirus IgM antibodies in human serum: Specific and non-specific reactions
✍ C. M. Nielsen; K. Hansen; H. M. K. Andersen; J. Gerstoft; B. F. Vestergaard πŸ“‚ Article πŸ“… 1987 πŸ› John Wiley and Sons 🌐 English βš– 558 KB

A mu-capture enzyme linked immunosorbent assay was developed for detection of IgM antibody to cytomegalovirus (CMV). Virus-specific IgM was detected using horseradish peroxidase labelled nuclear CMV antigen (CMV-ELA). False-positive reactions caused by Paul-Bunnell-Davidsohn (PBD) positive sera and