Sensitive detection of tumor marker CA15-3 in human serum by capillary electrophoretic immunoassay with chemiluminescence detection

✍ Scribed by Yan-Ming Liu; Yan-Li Zheng; Jun-Tao Cao; Yong-Hong Chen; Fu-Rong Li

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 448 KB
• Volume: 31
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200700590

✦ Synopsis

Abstract

A new and sensitive non‐competitive immunoassay (IA) for tumor marker carbohydrate antigen 15‐3 (CA15‐3) by CE coupling with ECL detection has been developed. This method is based on luminol–H~2~O~2~ reaction catalyzed by horseradish peroxidase (HRP). The optimum CE separation and CL detection conditions were investigated. After the non‐competitive immunoreaction, the free HRP‐labeled CA15‐3 antibody (Ab*) and the bound Ab*‐antigen (Ab*‐Ag) complex were separated in a separation capillary and then catalyzed the CL reaction of luminol and H~2~O~2~ in a reaction capillary following the separation capillary. The calibration curve based on the peak areas of Ab*‐Ag complex plotted against the concentrations of CA15‐3 is in the range of 0–250 U/mL with a correlation coefficient of 0.9983 and the detection limit is 0.035 U/mL (S/N = 3). The response for five consecutive injections of 125 U/mL CA15‐3 resulted in RSDs of 0.83% and 3.1% for the migration time and the peak area, respectively. The method was successfully used for the quantification of CA15‐3 in human sera obtained from healthy persons and from patients with breast cancer.