Use of Real-Time Polymerase Chain Reacti
β
Nathalie Y. Fortin; Ashok Mulchandani; Wilfred Chen
π
Article
π
2001
π
Elsevier Science
π
English
β 87 KB
Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t