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Detection of Escherichia coli O157 using equal-length double-stranded fluorescence probe in a real-time polymerase chain reaction assay

✍ Scribed by Guangfu Yu; Jianjun Niu; Mingshan Shen; Hanjuan Shao; Liang Chen


Book ID
116347044
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
247 KB
Volume
366
Category
Article
ISSN
0009-8981

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Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t