Detection of DNA strand breaks in brown trout (Salmo trutta) hepatocytes and blood cells using the single cell gel electrophoresis (comet) assay
β Scribed by C.L Mitchelmore; J.K Chipman
- Book ID
- 117347301
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 407 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0166-445X
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The aim of the reported study was to investigate the reproducibility of the single-cell gel electrophoresis (SCGE) assay in the determination of DNA singlestrand breaks (SS Bs) and to estimate the statistical requirements when the SCGE assay is used for the detection of genotoxicity in humans In hum
The alkaline single cell gel electrophoresis (SCGE) or "comet" assay under alkaline conditions was used to measure DNA damage in the liver cells of B6C3F1 male mice exposed to 2,4-dimethylaniline and 2,4,6-trimethylaniline. Cells embedded in agarose were lysed, subjected briefly to an electric field