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Detection of Campylobacter and Escherichia coli O157:H7 from filth flies by polymerase chain reaction

โœ Scribed by A. L. Szalanski; C. B. Owens; T. Mckay; C. D. Steelman


Book ID
110748980
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
195 KB
Volume
18
Category
Article
ISSN
0269-283X

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Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t