๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Detection of antibodies to central nervous system antigens by solid phase radioimmunoassay

โœ Scribed by Dr. D. Scott Linthicum; Ian R. Mackay; Anne Wilson; Leonie B. Horvath; Patrick R. Carnegie

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
649 KB
Volume
6
Category
Article
ISSN
0360-4012

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โœฆ Synopsis

Abstract

A solid phase radioimmunoassay is described which employs ^125^Iโ€proteinโ€A to detect the presence of antibodies against a panel of cellular and soluble central nervous system (CNS) specific antigens coated onto polyvinylchloride Microtiter plates. Serum antibodies from rabbits immunized against myelin, myelin basic protein (MBP), glial acidic fibrillary protein (GFAP), astroglioma cells, and cerebellar cells were easily detected, and high specificity for each antiserum and antigen was also demonstrable. The assay is applicable to cerebrospinal fluid (CSF) from patients with neurological diseases to detect antibodies against CNSโ€specific antigens. The assay should be useful for examining cell lines derived from CNS tissue for the presence of brain proteins.

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