✦ LIBER ✦

Detection and enrichment of disseminated renal carcinoma cells from peripheral blood by immunomagnetic cell separation

✍ Scribed by Udo Bilkenroth; Helge Taubert; Dagmar Riemann; Udo Rebmann; Hans Heynemann; Axel Meye

Publisher: John Wiley and Sons
Year: 2001
Tongue: French
Weight: 333 KB
Volume: 92
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.1217

No coin nor oath required. For personal study only.

✦ Synopsis

We have established an immunomagnetic separation procedure for the detection of circulating tumor cells in the peripheral blood based on the magnetic cell sorting (MACS) technique. In previous in vitro experiments, renal-cell carcinoma (RCC) cells were mixed with peripheral blood. In dilutions of 1:200 to 1:10 7 tumor cells per mononuclear blood cells, an average recovery rate of 84% of tumor cells was determined. In our study, 104 peripheral blood samples from 59 renal carcinoma patients were analyzed. MACS resulted in significant depletion of leukocytes, permitting a search for tumor cells on just 1 slide. Analyzing 8 ml of peripheral blood per patient, 19/59 RCC patients carried disseminated tumor cells (32%) in the range of 1 to 38 cells (median 8). Interestingly, for the cytokeratin-positive (CK ؉ ) patient group, we found a correlation between tumor cell number and grading (G2 vs. G3) and an increased number of CK ؉ patients with advanced tumor stage. MACS appears to be an efficient technique to detect disseminated tumor cells in peripheral blood.

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