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Detection and differentiation of sugars on paper by the p-anisidine/periodate reaction

✍ Scribed by L.A. Veiga; E.L. Chandelier

Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
235 KB
Volume
20
Category
Article
ISSN
0003-2697

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✦ Synopsis

During the past few years a considerable amount of work has been reported on the identification and detection of sugars and their deriv:\tivcs by paper chromatography and thin-layer chromatography (1-12 ).

The periodate/benzidine reagent has been used to differentiate polyol:: from other sugars (13-15) and Bean and Porter ( 14) observed that panisidinc could replace benzidine for t'he same purpose. p-Anisidincphosphoric acid has been found to be a satisfactory reagent for diffcrentiating several classes of sugars, and also for serving as a color reagent for sialic acid compounds on paper chromatogrnms (15? 16). p-hnisidine-I)llosphatc in ethanol has been used as spray reagent for sugars (17, 181.

Certain deoxy sugars and aldehydes can be detected I);-using periodatcs ositlation followed by thiobarbituric acid (19)(20)(21) This report describes a method for the detection and differentiation of lu:my sugars and sugar derivatives by paper chromatography, bascl(l on their reaction with p-anisidine and sodium pcriodatc.

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