Double-stranded DNA can be viewed as a multifunctional, modular receptor that can be read sequenceselectively in a digital way (base pair per base pair) by a complementary, similarly modular ligand. This principle has been exploited in several approaches to design sequence-specific DNA-binding ligan
โฆ LIBER โฆ
Design of novel sequence-specific DNA-binding proteins
โ Scribed by David J Segal; Carlos F Barbas III
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 238 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1367-5931
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We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble