Chemical Approaches Untangling Sequence-Specific DNA Binding by Proteins
β Scribed by Shin-ichi Sato; Masaki Hagihara; Kenji Sugimoto; Takashi Morii
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 283 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0947-6539
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We describe a rapid and sensitive method to isolate sets of high-affinity binding sites for sequence-specific DNA binding proteins. The DNA binding domain of the protein is expressed in Escherichia coli as a fusion with glutathione S-transferase (GST). Binding reactions are set up with total soluble