Derivatization with 1-Pyrenyldiazomethane Enhances Ionization of Glycopeptides but Not Peptides in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
โ Scribed by Amano, Junko; Nishikaze, Takashi; Tougasaki, Fumio; Jinmei, Hiroshi; Sugimoto, Ichiro; Sugawara, Shu-ichi; Fujita, Masaya; Osumi, Kenji; Mizuno, Mamoru
- Book ID
- 126833717
- Publisher
- American Chemical Society
- Year
- 2010
- Tongue
- English
- Weight
- 592 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0003-2700
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The limits of performance of a linear time-of-flight mass spectrometer using matrix-assisted laser desorp tionlionization have been tested. Effects of the pressure in the vacuum system and the sampling rate of the digital oscilloscopes on mass resolution have been investigated. Spectra recorded at d
One problem of matrix-assisted laser desorption ionization coupled to time-of-flight mass spectrometry is the moderate mass accuracy that typically can be obtained in routine applications, Here we report improved mass accuracy for peptides, even when low amounts and complex peptide mixtures are used