✦ LIBER ✦

Derivatization of small biomolecules for optimized matrix-assisted laser desorption/ionization mass spectrometry

✍ Scribed by Andreas Tholey; Christoph Wittmann; Min-Jung Kang; Ditte Bungert; Klaus Hollemeyer; Elmar Heinzle

Publisher: John Wiley and Sons
Year: 2002
Tongue: English
Weight: 167 KB
Volume: 37
Category: Article
ISSN: 1076-5174
DOI: 10.1002/jms.355

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✦ Synopsis

Abstract

Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) is a powerful tool for the measurement of low molecular mass compounds of biological interest. The limitations for this method are the volatility of many analytes, possible interference with matrix signals or bad ionization or desorption behavior of the compounds. We investigated the application of well‐known and straightforward one‐pot derivatization procedures to circumvent these problems. The derivatizations tested allow the measurement and the labeling of alcohols, aldehydes and ketones, carboxylic acids, α‐ketocarboxylic acids and amines. Copyright © 2002 John Wiley & Sons, Ltd.

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