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Density Labeling of Mammalian Cell DNA and RNA with Nitrogen-15

โœ Scribed by N. A. Crane and N. P. Salzman

Book ID
123648514
Publisher
National Academy of Sciences
Year
1968
Tongue
English
Weight
741 KB
Volume
61
Category
Article
ISSN
0027-8424

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We describe methods to introduce 13 C specifically to the C2 or C8 positions of 15 N-multilabeled purines, thereby permitting " 13 C tagging" of all base nitrogens except the amino group of adenosine. These procedures permit the incorporation of two or more 15 N-multilabeled monomers into a given DN