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Metabolic labeling of RNA uncovers principles of RNA production and degradation dynamics in mammalian cells

โœ Scribed by Rabani, Michal; Levin, Joshua Z; Fan, Lin; Adiconis, Xian; Raychowdhury, Raktima; Garber, Manuel; Gnirke, Andreas; Nusbaum, Chad; Hacohen, Nir; Friedman, Nir


Book ID
111880331
Publisher
Nature Publishing Group
Year
2011
Tongue
English
Weight
963 KB
Volume
29
Category
Article
ISSN
1087-0156

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Metabolism of U6 RNA species in nonirrad
โœ Kanakendu Choudhury; Indrani Choudhury; Robert W. Jones; Chellaiah Thirunavukkar ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 942 KB

We observed a series of rapidly labeled U6 KNA bands, which were hybrid selected with U6 DNA, in nonirradiated human cells. The electrophoretic mobility of these bands in denaturing gels was lower than that of the known mature U6 RNA species, and was equivalent to transcripts up to -7 nucleotides lo