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Deletion mapping of chromosome 19 in human gliomas

✍ Scribed by Andreas von Deimling; Judith Nagel; Bernhard Bender; Doris Lenartz; Johannes Schramm; David N. Louis; Otmar D. Wiestler

Publisher: John Wiley and Sons
Year: 1994
Tongue: French
Weight: 762 KB
Volume: 57
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910570511

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

There is evidence that a putative glioma tumor suppressor locus resides on the long arm of chromosome 19. We present data on 161 gliomas from IS6 patients, which were studied by microsatellite analysis for loss of heterozygosity (LOH) on chromosome 19. Eight loci on the long arm and 2 loci on the short arm of chromosome IV were examined. LOH on I9qwas observed in 3/19 astrocytomas (WHO grade II), 12/27 anaplastic astrocytomas (WHO grade III), 16/76 cases of glioblastoma multiforme WHO (grade IV), 4/9 oligodendrogliomas (WHO grade II), 3/5 anaplastic oligodendrogliomas (WHO grade III), 5/9 mixed oligo‐astrocytomas (WHO grade II) and 8/10 anaplastic oligo‐astrocytomas (WHO grade III). While 31 of the tumors with LOH on chromosomal arm I9q exhibited allelic loss at every informative locus, 20 tumors showed terminal or interstitial deletions. In contrast to astrocytomas and glioblastomas, tumors with an oligodendroglial component had predominantly lost the entire long arm of chromosome 19. The common region of overlap in gliomas was located on 19q 13.2‐q 13.4 between the markers D 19S 178 and D 19S 180. Our data confirm the involvement of a putative tumor suppressor gene on chromosomal arm 19q in gliomas and assign this gene to 19q 13.2‐q 13.4.

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