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Degree of exposure of membrane proteins determined by fluorescence quenching

✍ Scribed by Shinitzky, M.; Rivnay, B.


Book ID
126989582
Publisher
American Chemical Society
Year
1977
Tongue
English
Weight
581 KB
Volume
16
Category
Article
ISSN
0006-2960

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Determination of proteins by fluorescenc
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Erythrosin B (EB) binding to proteins causes a decrease in the fluorescence maximum of EB at 550nm. Based on this, a new, fast and simple fluorescence quenching method for the determination of proteins is developed. The linear range of this assay is 1.36-20.4pgmlK'. The method has very few interfer