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Determination of proteins by fluorescence quenching of erythrosin B

✍ Scribed by Chun Qi Ma; Ke An Li; Shen Yang Tong

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
434 KB
Volume
333
Category
Article
ISSN
0003-2670

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✦ Synopsis

Erythrosin B (EB) binding to proteins causes a decrease in the fluorescence maximum of EB at 550nm. Based on this, a new, fast and simple fluorescence quenching method for the determination of proteins is developed. The linear range of this assay is 1.36-20.4pgmlK'.

The method has very few interferences, most of which can be minimized by dilution. In the detection of proteins in human serum, this method gave values close to that of the Coomassie brilliant blue method.

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