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Determining the Membrane Topology of Peptides by Fluorescence Quenching †

✍ Scribed by Wimley, William C.; White, Stephen H.


Book ID
120465244
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
154 KB
Volume
39
Category
Article
ISSN
0006-2960

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The transverse organisation of ubiquinon
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The transverse organisation of ubiquinone in mitochondrial membranes was investigated by quenching a set of fluorescent fatty acids. We show that the fluorescent moiety of the probes is located at a graded series of depths in the mitochondrial membrane. The probes sense the characteristics of the li