Degradation of isotopically labeled citric, α-ketoglutaric and glutamic acids
✍ Scribed by E.H. Mosbach; E.F. Phares; S.F. Carson
- Book ID
- 118839078
- Publisher
- Elsevier Science
- Year
- 1951
- Tongue
- English
- Weight
- 373 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0003-9861
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## Abstract We have developed methods for the preparation of L‐glutamic acid, isotopically labeled with ^13^C, using commercially available purified enzymes. The procedure is sufficiently versatile that L‐glutamic acid may be labeled at any carbons or any combination of carbons using ^13^C‐labeled
The syntheses of a-ketoC2-' 3C]glutaric acid and a-ketoC1,2-' 'Cl glutaric acid were each achieved from commercial "CH,I in 5 steps. CH,NO,, prepared in situ from "CH,I and AgNO,, was treated with magnesium methyl carbonate, followed by acidified methanol to give 0,N13CH,C0,CH3. Michael addition to