✦ LIBER ✦

Deaminating enzyme labels for potentiometric enzyme immunoassay

✍ Scribed by C.R. Gebauer; G.A. Rechnitz

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 876 KB
Volume: 124
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90049-5

No coin nor oath required. For personal study only.

✦ Synopsis

Adenosine deaminase, asparaginase, and urease are examined as possible enzyme labels for immunoassays using potentiometric detection with the ammonia gas-sensing membrane electrode. Considerations of binding ability, retained activity, and stability reveal asparaginase to be the most effective enzyme label for immunoassay purposes. The utility of the potentiometric approach with this enzyme label is demonstrated via model hapten assays for dinitrophenyl groups and for cortisoi.

📜 SIMILAR VOLUMES

Enzyme immunoassay technique using alkal

Enzyme immunoassay technique using alkaline phosphatase enzyme labels and a Nafion electrode as sensor

✍ C. Degrand; B. Limoges; S. Rapicault 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 298 KB

Enzyme immunoassay for captopril

✍ H. Kinoshita; R. Nakamaru; S. Tanaka; Y. Tohira; M. Sawada 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 296 KB

New immunoassay techniques using Nafion-

New immunoassay techniques using Nafion-modified electrodes and cationic redox labels or enzyme labels

✍ A. Le Gal La Salle; B. Limoges; S. Rapicault; C. Degrand; P. Brossier 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 596 KB

Two new competitive immunoassay techniques with sensitive electrochemical detection at Nafion film modified electrodes are described and compared. Method A implies the use of an antigen labeled with a cationic redox group in homogeneous immunoassay. Method B involves an enzyme-labeled antigen, in co

An enzyme immunoassay for secretin

✍ Hiroshi Tanaka; Kouichi Katayama 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 546 KB

A sensitive and specific enzyme immunoassay for secretin was developed with the use of enzyme-labeled antigens. Synthetic porcine secretin and its carboxy-terminal fragments (residues 11-27 and 18-27) were conjugated with beta-D-galactosidase for use in the immunoassay, and the assay method with the

Bioluminescent enzyme immunoassay for pr

Bioluminescent enzyme immunoassay for progesterone using monoclonal antibodies and glucose-6-phosphate dehydrogenase labels

✍ Brochu, Michèle ;Carrié, Marie-Luce ;Térouanne, Béatrice ;Bélanger, Alain ;Nicol 📂 Article 📅 1987 🏛 John Wiley and Sons ⚖ 577 KB

Bacterial luciferase, NAD( P): FMN oxidoreductase and anti-mouse immunoglobulin were co-immobilized on Sepharose 4B. This reagent together with a progesterone glucose-6phosphate dehydrogenase conjugate and various anti-progesterone monoclonal antibodies was used t o develop a non-separation biolumin

Homogeneous enzyme immunoassay for macro

Homogeneous enzyme immunoassay for macromolecular antigens using avidin biotin enzyme

✍ Yoshihiro Ashihara; Isao Nishizono; Eiich Miyagawa; Yasushi Kasahara 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 244 KB

A unique homogeneous enzyme immunoassay used for the determination of haptens and macromolecular antigens has been developed. It consists of avidin-antigen conjugate, anti-ligand antibody, and biotin enzyme (propionyl-CoA carboxylase). The immunological reaction of the conjugate with antibodies spec