An enzyme immunoassay for secretin

✍ Scribed by Hiroshi Tanaka; Kouichi Katayama

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 546 KB
• Volume: 139
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90404-4

✦ Synopsis

A sensitive and specific enzyme immunoassay for secretin was developed with the use of enzyme-labeled antigens. Synthetic porcine secretin and its carboxy-terminal fragments (residues 11-27 and 18-27) were conjugated with beta-D-galactosidase for use in the immunoassay, and the assay method with the latter fragment (residues 18-27) linked to beta-D-galactosidase was found to be the most sensitive. The minimum amount of secretin detectable by this method was 1-2.5 pg/assay. Serum levels of secretin after intravenous injection of the peptide in rats were determined by both the enzyme immunoassay and a commercial radioimmunoassay kit. The correlation coefficient between the levels measured by the two methods was 0.984. The enzyme immunoassay could detect immunoreactive secretin levels in normal human sera, giving a value of 16.9 +/- 2.2 pg/ml (mean +/- SE of six human subjects).