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Cytosolic phospholipase A2 activity during the ongoing cell cycle

✍ Scribed by Gerda S.A.T. van Rossum; Angela S. Vlug; Henk van den Bosch; Arie J. Verkleij; Johannes Boonstra

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 168 KB
Volume: 188
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1123

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✦ Synopsis

Cytosolic phospholipase A(2) (cPLA(2)) is of special interest because it selectively releases arachidonic acid from membrane phospholipids. Arachidonic acid has been implicated to play an important role in various cellular responses. Recently arachidonic acid release and prostaglandin synthesis have been shown to be cell cycle dependent and therefore the activity of cPLA(2) during the ongoing cell cycle was investigated, using the mitotic shake off method for cell synchronisation. cPLA(2) activity was high in mitotic cells and decreased rapidly in the early G1 phase. A strong increase in activity was measured following the G1/S transition in both neuroblastoma and Chinese hamster ovary cells. The changes in activity were not due to a difference in cPLA(2) expression but due to phosphorylation of cPLA(2). Phosphorylation of cPLA(2) occurs through MAPK since the use of a specific MAPK kinase inhibitor and serum depletion of synchronised cells inhibited cPLA(2) activity.

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