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Cytolytic activity of interferon-gamma and its synergism with 5-fluorouracil

✍ Scribed by Junming Le; Yum K. Yip; Jan Vilček

Publisher
John Wiley and Sons
Year
1984
Tongue
French
Weight
687 KB
Volume
34
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Highly purified natural or recombinant human immune interferon (IFN-gamma) was found to be directly cytolytic to certain tumor cell lines in v i t m Out of 5 human tumor cell lines and one normal fibroblast line tested, the colon adenocarcinoma line HT-29 and the rhabdomyosarcoma line A673 were highly sensitive to cytolysis by interferon, as determined by 'UI-iododeoxyuridine release in a 72 h microcytotoxicity assay. Cytolysis was marked at IFNgamma concentrations of less than I Ulml, and it reached a near-maximal level at 6.4 Ulml. A synergistic cytolysis on HT-29 cells of IFN-gamma and 5-fluorouracil (5-FU) was observed at 5-FU concentrations ranging from 64 to 640pg/ ml. In contrast, no synergism was observed between IFNgamma and mitomycin C. The direct cytolytic activity and synergistic cytolysis with 5-FU of the IFN-gamma preparations used in the present study were abolished completely by treatment with a neutralizing monoclonal antibody specific for human IFN-gamma.

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