Measurement of 5-fluorouracil and its active metabolites in tissue

✍ Scribed by Theodore A. Stein; Gerard P. Burns; Beverly Bailey; Leslie Wise

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 494 KB
• Volume: 507
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84203-5

✦ Synopsis

The Sfluorouracil content of serum, bile, pancreatic juice, liver, pancreas and muscle was measured by reversed-phase high-performance liquid chromatography using a mobile phase of 5 mM 1-heptanesulfonic acid in 5 mM acetic acid. Free or unmetabolized Sfluorouracil was extracted from samples with a mixture of light petroleum-n-propanol (40:60). The active metabolites of 5-fluorouracil were hydrolyzed with hot perchloric acid to free 5-fluorouracil and the combined 5-fluorouracil content was extracted. The active metabolite fraction was calculated from the difference between the combined and the free fractions. A straight line plot of the peak areas against concentration was achieved and the detection limit was 50 ng/ml. Five minutes after stopping an intravenous infusion of 15 mg/kg of 5-fluorouracil in a dog, the serum contained only the free form, but other body fluids and tissues contained both free and metabolite fractions. The method may be useful to determine the amount of total drug in patient samples. '1'. A. S'I'EIN et al.