## Abstract Cytochemical studies of adenylate cyclase activity were performed on __Dugesia lugubris s.l.__ to ascertain which cell types, among the heterogenous cell population, showed significant adenylate cyclase activity on their plasma membranes. Nondecapitated worms and cephalic regenerants w
Cytochemical methods for the localization of invertase activity in plant tissues
โ Scribed by J. Chapman; A. McGarry; D. F. Carmignac; P. D. Gahan
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 618 KB
- Volume
- 90
- Category
- Article
- ISSN
- 1432-119X
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โฆ Synopsis
Two cytochemical methods for the localization of acid and alkaline invertases are given. The first is based upon the reduction of a silver complex at two different pH ranges, whilst the second is based upon the tetrazolium reaction and permits quantification of the rate of activity of alkaline invertase activity. The distribution of alkaline invertase activity throughout the root apex of Pisum sativum and the cell wall localization of acid invertase for material excised from tuber tissue of Helianthus tuberosus are both confirmed.
๐ SIMILAR VOLUMES
A quantitative cytochemical method for the demonstration of phosphofructokinase has been successfully applied to a range of plant tissues. The findings indicate that this enzyme system may be assayed as an indicator of glycolytic activity in plant cells, and furthermore tha the very high endogenous