To characterize the vasopressin-adenylate cyclase (AC) signaling pathway in control of Na Ο© reabsorption in cultured renal (A6) cells, we determined the distribution of AC with a cytochemical technique using 5Π-adenylylimidodiphosphate as substrate and cerium chloride as capturing agent. The additio
Cytochemical localization of adenylate cyclase activity in planarian tissues
β Scribed by Pascolini, Rita ;Gargiulo, A. M. ;Spreca, A. ;Ceccarelli, P.
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 454 KB
- Volume
- 223
- Category
- Article
- ISSN
- 0022-104X
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β¦ Synopsis
Abstract
Cytochemical studies of adenylate cyclase activity were performed on Dugesia lugubris s.l. to ascertain which cell types, among the heterogenous cell population, showed significant adenylate cyclase activity on their plasma membranes.
Nondecapitated worms and cephalic regenerants were examined in replicated experiments. Evidence of adenylate cyclase activity was noted in secretory tissues and the protonephridial system of nonβoperated worms, and also in dedifferenβtiating and regenerating tissues of operated worms. Observations presented in this study call attention to the role of adenylate cyclase in some of the main physiological processes of triclads, such as secretion, excretion, and regeneration.
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Previous investigation has provided evidence for the control of electrogenic chloride secretion by pituitary adenylate cyclase-activating polypeptide (PACAP) across the rat epididymal epithelium using electrophysiological measurement of transepithelial transport in cultured epididymal system. Hence,
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