Two cytochemical methods for the localization of acid and alkaline invertases are given. The first is based upon the reduction of a silver complex at two different pH ranges, whilst the second is based upon the tetrazolium reaction and permits quantification of the rate of activity of alkaline inver
A quantitative cytochemical method for phosphofructokinase in plant tissues
β Scribed by P. B. Gahan; L. M. Bellani; M. A. Rana; B. D. Lake
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 277 KB
- Volume
- 78
- Category
- Article
- ISSN
- 1432-119X
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β¦ Synopsis
A quantitative cytochemical method for the demonstration of phosphofructokinase has been successfully applied to a range of plant tissues. The findings indicate that this enzyme system may be assayed as an indicator of glycolytic activity in plant cells, and furthermore tha the very high endogenous phosphoenolpyruvate concentrations may not be rate limiting in vivo.
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## Thin-Layer Chromatographic Separation and Analysis of @'-Labeled Compounds1 The isotopic analysis of oxygen in organic compounds requires the use of fairly large samples for conversion by various methods (1,2) to carbon