Cycloheximide resistance in Chinese hamster cells: II. Induction of CHM resistance in Chinese hamster cells by N-nitrosomethylurea
✍ Scribed by Hubert Pöche; Nina B. Varshaver; Michael Theille; Erhard Geissler
- Book ID
- 113190712
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 294 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0027-5107
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Cycloheximide(CHM)-resistant mutant Chinese hamster ovary (CHO) and human cells were induced with N-nitrosomethylurea (NMU) and ethyl methanesulfonate (EMS); the mutants were viable and showed unlimited growth in the presence of CHM (7 X 10(-7) M), whereas this concentration inhibits protein synthes
## Abstract Previous studies from our laboratory have shown that Chinese hamster V79 cells mutated to high level resistance to amphotericin B have a lower cellular level of cholesterol, the target molecule for the polyene antibiotic. Two amphotericin B‐resistant (AMB^R^) mutants were each hybridize