Amphotericin B resistance is recessive in Chinese hamster hybrid cells

✍ Scribed by Katsuhiko Hidaka; Shin-Ichi Akiyama; Michihiko Kuwano

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 486 KB
• Volume: 106
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041060106

✦ Synopsis

Abstract

Previous studies from our laboratory have shown that Chinese hamster V79 cells mutated to high level resistance to amphotericin B have a lower cellular level of cholesterol, the target molecule for the polyene antibiotic. Two amphotericin B‐resistant (AMB^R^) mutants were each hybridized to their parental amphotericin B‐sensitive (AMB^S^) V79 cells. All the hybrids derived from AMB^R^/AMB^S^ fusions were as sensitive to polyene antibiotics (amphotericin B, filipin, and pimaricin) as AMB^S^/AMB^S^ hybrids. The AMB^R^/AMB^S^ hybrids were found to contain cholesterol per phospholipids that is comparable to those in AMB^S^ or AMB^S^/AMB^S^. The analysis of hybrids formed between mutant and wild‐type cells thus indicated that resistance to amphotericin B is a recessive marker, and that the cellular level of cholesterol is compensated in the AMB^S^/AMB^R^ hybrids. Hybrids of AMB^R^ and AMB^R^ cells were all resistant, so that the three AMB^R^ mutants all fell into a single complementation group.