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Current Protocols in Protein Science || High-Throughput Screening for Protein-Protein Interactions Using Yeast Two-Hybrid Arrays

✍ Scribed by Coligan, John E.; Dunn, Ben M.; Speicher, David W.; Wingfield, Paul T.


Book ID
120210594
Publisher
Wiley (John Wiley & Sons)
Year
2001
Tongue
English
Weight
286 KB
Volume
24
Category
Article
ISBN-13
9780471140863
ISSN
1934-3655

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✦ Synopsis


Scientists across disciplines have increasingly come to recognize the power of the protein. Current Protocols in Protein Science on CD-ROM (CPPS on CD) was developed in response to this revitalized interest and provides the most comprehensive collection of expert protein methods available. The publication covers both basic and advanced methods used in protein purification, characterization, and analysis as well as post-translational modification and structural analysis. More than 800 basic, support and alternate protocols have been carefully chosen for maximum applicability. CPPS on CD contains the complete contents of the corresponding two-volume looseleaf with the added benefits of a quick and intuitive search engine and user interface. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Quarterly update discs keep the set current with the latest developments in genome methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Human Genetics, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.


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## Abstract The analysis of protein‐protein interactions is a key focus of proteomics efforts. The yeast two‐hybrid system (Y2H) has been the most commonly used method in genome‐wide searches for protein interaction partners. However, the throughput of the current yeast two‐hybrid array approach is