Current Protocols in Molecular Biology || Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy

✍ Scribed by Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin

• Book ID: 120639415
• Publisher: Wiley (John Wiley & Sons)
• Year: 2006
• Tongue: English
• Weight: 571 KB
• Volume: 76
• Category: Article
• ISBN-13: 9780471142720
• ISSN: 1934-3639
• DOI: 10.1002/0471142727.mba03ds76

✦ Synopsis

Abstract

Quantitation of nucleic acids is a fundamental tool in molecular biology that requires accuracy, reliability, and the use of increasingly smaller sample volumes. This unit describes the traditional absorbance measurement at 260 nm and three more sensitive fluorescence techniques, as well as three microvolume methods that use fiber optic technology in specialized cells or instrumentation. These procedures allow quantitation of DNA solutions ranging from 1 pg/l to 50 mg/ml.