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Current Protocols in Human Genetics || Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy

โœ Scribed by Haines, Jonathan L.; Korf, Bruce R.; Morton, Cynthia C.; Seidman, Christine E.; Seidman, J.G.; Smith, Douglas R.

Book ID
120507342
Publisher
Wiley (John Wiley & Sons)
Year
2007
Tongue
English
Weight
399 KB
Volume
53
Category
Article
ISBN-13
9780471142904
ISSN
1934-8266

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Current Protocols in Molecular Biology |
Current Protocols in Molecular Biology || Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
โœ Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidm ๐Ÿ“‚ Article ๐Ÿ“… 2006 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 571 KB

## Abstract Quantitation of nucleic acids is a fundamental tool in molecular biology that requires accuracy, reliability, and the use of increasingly smaller sample volumes. This unit describes the traditional absorbance measurement at 260 nm and three more sensitive fluorescence techniques, as wel