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Current Protocols in Immunology || Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy

โœ Scribed by Coligan, John E.; Bierer, Barbara E.; Margulies, David H.; Shevach, Ethan M.; Strober, Warren

Book ID
120811041
Publisher
John Wiley & Sons, Inc.
Year
2001
Weight
204 KB
Category
Article
ISBN-13
9780471142737

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Current Protocols in Molecular Biology |
Current Protocols in Molecular Biology || Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
โœ Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidm ๐Ÿ“‚ Article ๐Ÿ“… 2006 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 571 KB

## Abstract Quantitation of nucleic acids is a fundamental tool in molecular biology that requires accuracy, reliability, and the use of increasingly smaller sample volumes. This unit describes the traditional absorbance measurement at 260 nm and three more sensitive fluorescence techniques, as wel