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Current Protocols in Cell Biology || Measurement of Oxidatively-Induced Clustered DNA Lesions Using a Novel Adaptation of Single Cell Gel Electrophoresis (Comet Assay)

✍ Scribed by Bonifacino, Juan S.; Dasso, Mary; Harford, Joe B.; Lippincott-Schwartz, Jennifer; Yamada, Kenneth M.


Book ID
120238622
Publisher
John Wiley & Sons, Inc.
Year
2010
Weight
405 KB
Category
Article
ISBN-13
9780471143031

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The alkaline single cell gel electrophoresis (SCGE) or "comet" assay under alkaline conditions was used to measure DNA damage in the liver cells of B6C3F1 male mice exposed to 2,4-dimethylaniline and 2,4,6-trimethylaniline. Cells embedded in agarose were lysed, subjected briefly to an electric field