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Crystallization and preliminary X-ray studies of human vascular anticoagulant protein

✍ Scribed by Anita Lewit-Bentley; Sylvie Doublié; Roger Fourme; Gerhard Bodo


Book ID
118918781
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
178 KB
Volume
210
Category
Article
ISSN
0022-2836

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Human procathepsin L has been expressed in the yeast Pichiapastoris and its inactive (Cys25Ser) and unglycosylated (ThrllOAla) mutant purified, concentrated to 4 mglml, and crystallized by vapor diffusion against solution containing 1.4 M (Na,K)PO, buffer, pH 7.8. Crystal size was increased by multi