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Crystallization and Preliminary X-ray Studies of Human Erythrocyte Acylpeptide Hydrolase

✍ Scribed by Michael Feese; Andrea Scaloni; Wanda M. Jones; James M. Manning; S.James Remington


Book ID
115626197
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
280 KB
Volume
233
Category
Article
ISSN
0022-2836

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Crystallization and preliminary x-ray di
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Human procathepsin L has been expressed in the yeast Pichiapastoris and its inactive (Cys25Ser) and unglycosylated (ThrllOAla) mutant purified, concentrated to 4 mglml, and crystallized by vapor diffusion against solution containing 1.4 M (Na,K)PO, buffer, pH 7.8. Crystal size was increased by multi