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Crystallization and preliminary X-ray diffraction studies of bacterial ribosomal protein L14

✍ Scribed by Davies, C. ;Gerchman, S. E. ;Kycia, J. H. ;McGee, K. ;Ramakrishnan, V. ;White, S. W.

Book ID
114529121
Publisher
International Union of Crystallography
Year
1994
Tongue
English
Weight
900 KB
Volume
50
Category
Article
ISSN
0907-4449

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Human procathepsin L has been expressed in the yeast Pichiapastoris and its inactive (Cys25Ser) and unglycosylated (ThrllOAla) mutant purified, concentrated to 4 mglml, and crystallized by vapor diffusion against solution containing 1.4 M (Na,K)PO, buffer, pH 7.8. Crystal size was increased by multi