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Crystallization and preliminary X-ray analysis of Pac17 from the pacidamycin-biosynthetic cluster of Streptomyces coeruleorubidus

✍ Scribed by Tromans, Daniel R. ;Stevenson, Clare E. M. ;Goss, Rebecca J. M. ;Lawson, David M.

Book ID: 117860177
Publisher: John Wiley and Sons
Year: 2012
Tongue: English
Weight: 758 KB
Volume: 68
Category: Article
ISSN: 1744-3091
DOI: 10.1107/s1744309112029144

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## Abstract A recombinant form of __Rhodobacter sphaeroides__ phosphoribulokinase (PRK), expressed in __Escherichia coli__ and isolated by affinity chromatography, was crystallized by the sitting drop vapor diffusion technique using NH~4~H~2~PO~4~ (pH 5.6) as the precipitating agent. PRK crystalliz