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Cryopreservation of adult human hepatocytes obtained from resected liver biopsies

✍ Scribed by E Alexandre; C Viollon-Abadie; P David; A Gandillet; P Coassolo; B Heyd; G Mantion; P Wolf; P Bachellier; D Jaeck; L Richert

Book ID
117088711
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
269 KB
Volume
44
Category
Article
ISSN
0011-2240

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A technique is described for isolation and culture of adult human hepatocytes from surgical liver biopsies. The mean cell yield was 1.75 X 10(7) cells per gm liver and viability averaged 80%. Hepatocytes were maintained in primary culture for about 10 days. Cell morphology and histochemical characte