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Comparison of cultured human hepatocytes isolated from surgical biopsies or cold-stored organ donor livers

✍ Scribed by Vons, C.; Pegorier, J.P.; Ivanov, M.A.; Girard, J.; Melcion, C.; Cordier, A.; Franco, D.


Book ID
123589011
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
297 KB
Volume
4
Category
Article
ISSN
0887-2333

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✍ Francois Ballet; Marie-Elisabeth Bouma; Shu-Ren Wang; Norma Amit; Jacqueline Mar 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 788 KB

A technique is described for isolation and culture of adult human hepatocytes from surgical liver biopsies. The mean cell yield was 1.75 X 10(7) cells per gm liver and viability averaged 80%. Hepatocytes were maintained in primary culture for about 10 days. Cell morphology and histochemical characte