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Cryo-electron microscopy of vitrified specimens: An approach to the study of bulk specimens

โœ Scribed by Erk, I. ;Delacroix, H. ;Nicolas, G. ;Ranck, J.-L. ;Lepault, J.

Publisher
Wiley (John Wiley & Sons)
Year
1991
Tongue
English
Weight
746 KB
Volume
18
Category
Article
ISSN
0741-0581

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โœฆ Synopsis

We are using and developing cryo-electron microscopy of vitrified specimens. Our main interests concern the structure of muscle and muscular components. Micrographs which generally contain periodic features are analyzed by numerical image processing methods. To detect artifacts induced by the electron microscopy techniques, we correlate our results to those obtained by X-ray diffraction. In this paper, we describe our approach to the study of bulk specimens. Vitrification of such specimens is assessed by cryo-X-ray diffraction. Microscopy is done on cryosubstituted specimens.

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