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Section directed cryosectioning of specimens for scanning electron microscopy: A new method to study cardiac development

✍ Scribed by Jeong-Wook Seo; Eul-Kyeong Kim; Nigel A. Brown; Andy Wessels

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
699 KB
Volume
30
Category
Article
ISSN
1059-910X

No coin nor oath required. For personal study only.

✦ Synopsis

A new method to study the developing heart was developed. Using this "section directed" cryosectioning method, appropriate fixed embryos can be trimmed optimally to obtain sectional planes that, if necessary, can be matched with histologically treated sections. As a result, the morphological information obtained with the scanning electron microscope can be compared in detail with the information on the molecular phenotypes of the subpopulations of cells as deducted from staining patterns of the sections. This method allows combination of the specific advantages of sophisticated histological techniaues. such as immunohistochemistry and in situ hybridisation,

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with-those of the scanning electron microscope.

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