We are using and developing cryo-electron microscopy of vitrified specimens. Our main interests concern the structure of muscle and muscular components. Micrographs which generally contain periodic features are analyzed by numerical image processing methods. To detect artifacts induced by the electr
Cryo-electron microscopy of vitrified aqueous specimens
β Scribed by Y. Talmon
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 100 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0304-3991
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Evaporation of water cannot be fully avoided when a n unsupported thin vitrified film of a n aqueous suspension is prepared for cryo-electron microscopy. This results in increasing concentration of solute which could affect the observed material. We have quantitatively studied this effect by measuri
Dimensional protein crystals imaged at low temperature and under low dose conditions reveal information to better than 3A resolution. Imaging under similar conditions and image reconstruction of non periodic specimens such as spherical viruses have yielded structural detail to only 30A resolution.