The procedure of either reversible or stable "zero-length" crosslinking of histones through their lysine residues directly to DNA partly depurinated under mild conditions at neutral pH is described. DNA in chromatin was methylated with dimethyl sulfate. Methylated purine bases in DNA can be readily
โฆ LIBER โฆ
Crosslinking of methylated DNA by moderate heating at neutral pH
โ Scribed by J. Burnotte; W.G. Verly
- Book ID
- 115745079
- Publisher
- Elsevier Science
- Year
- 1972
- Weight
- 228 KB
- Volume
- 262
- Category
- Article
- ISSN
- 0005-2787
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The method of crosslinking histones to D
โ
E.S. Levina; S.G. Bavykin; V.V. Shick; A.D. Mirzabekov
๐
Article
๐
1981
๐
Elsevier Science
๐
English
โ 880 KB
Resistance of alkylated DNA to degradati
โ
P.J. O'Connor; J.G. Salisbury; G.P. Margison
๐
Article
๐
1976
๐
Elsevier Science
๐
English
โ 865 KB
Heat-induced denaturation and aggregatio
โ
Mireille Weijers; Peter A. Barneveld; Martien A. Cohen Stuart; Ronald W. Vissche
๐
Article
๐
2003
๐
Cold Spring Harbor Laboratory Press
๐
English
โ 194 KB
Enhancing DNA Triple Helix Stability at
โ
Dipl.-Chem. Stefan Hildbrand; Prof. Christian Leumann
๐
Article
๐
1996
๐
John Wiley and Sons
๐
English
โ 373 KB
over MgSO, and concentrated under reduced pressure. After separation by column chromatography (silica gel, hexane:EtOAc = 20:1), 12b (20mg. 0.11 mmol) and 13b (56 mg, 0.30 mmol) were isolated in 21 and 60% yield, respectively. as colorless oils.12b;'HNMR(30OMHz,CDCI3):6 =1.03(s,3H).2.19(dd,J=l5.0,7.
Effects of heating at neutral and acid p
โ
Ritsuko Wada; Yuki Fujita; Naofumi Kitabatake
๐
Article
๐
2006
๐
Elsevier Science
๐
English
โ 289 KB
Light-Scattering Study of the Structure
โ
Mahmoudi, Najet; Mehalebi, Soraya; Nicolai, Taco; Durand, Dominique; Riaublanc,
๐
Article
๐
2007
๐
American Chemical Society
๐
English
โ 121 KB