Enhancing DNA Triple Helix Stability at Neutral pH by the Use of Oligonucleotides Containing a More Basic Deoxycytidine Analog
✍ Scribed by Dipl.-Chem. Stefan Hildbrand; Prof. Christian Leumann
- Book ID
- 101556754
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 373 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0044-8249
No coin nor oath required. For personal study only.
✦ Synopsis
over MgSO, and concentrated under reduced pressure. After separation by column chromatography (silica gel, hexane:EtOAc = 20:1), 12b (20mg. 0.11 mmol) and 13b (56 mg, 0.30 mmol) were isolated in 21 and 60% yield, respectively. as colorless oils.12b;'HNMR(30OMHz,CDCI3):6 =1.03(s,3H).2.19(dd,J=l5.0,7.1 Hz, 1 H). 2.37 (dd. J ~1 5 . 0 . 7 . 5 Hz. 1 H),2.75, 2.88 (d. J =14.3 Hz. 2H), 5.10 (m, 2H), 5.74 (m. 1 H), 7.05-7.35 (m. 5H). 9.60 (s, 1 H). 13b (55:45 mixture of diastereoisomers).'HNMR(300MHr.CDCI,):6=0.88,1.10(d,J=7.1 Hz,3H). 1.19,1.25 (s.3H). 1.32. 1.65(dd, J=10.5. 7.1 Hz, 1 H).1.94.2.32,(dd, J=10.4.9.7Hz. 1H). 2.65.2.72.2.91.2.97(d,J=14.3Hz.2H),2.77,3.29(m,1H).7.10-7.35(m,5H); "C NMR (300 MHz. CDCI,): d = 13.00