Crabtree effect in some bacterial cultures
β Scribed by I. Mustea; T. Muresian
- Publisher
- John Wiley and Sons
- Year
- 1967
- Tongue
- English
- Weight
- 219 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0008-543X
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β¦ Synopsis
The Gabtree effect, considered an inherent peculiarity of the majority of malignant tissues, was found in all examined strains of Escherichia and Proteus and was absent in Micrococcus and Serratia. The maximal value of the Crabtree effect in E. coli cultures occurred at the time of the initial period of log multiplication. The finding of the Crabtree effect in the examined bacteria strains indicates an analogy existing between the metabolic behavior of malignant tissues and certain facultatively anaerobic bacteria.
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The occurrence of a Crabtree effect in HeLa cells was detected. Some properties of pyruvate kinase (PK) were also evaluated. Hexose phosphate, triose-phosphate and phosphoenolpyruvate (PEP) significantly decreased the oxygen consumption of digitonin-permeabilized HeLa cells, which were oxidizing suc
The cessation of growth in bacterial cultures has been attributed to the depletion of some nutrient factor or factors essential to the anabolic processes of the cells (e.g., Barnes, '31; Jordan and Jacobs, '44; Lockhart and Powelson, '53a). By studying cultures in which total growth was limited by a